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(i) Mechanical disturbance: training is followed by shaking (red springs) animals in lower viscosity food every 15 min for 2 h from 0–2, 2–4, or 4–6 h after training. Figure S3 indicates that animals eat during mechanical disturbance. Videos of sleep disruption are in Video S3, related to Figure 2. After disturbance, animals are recovered on food without shaking until 16 h after training. (ii) Metabolic disturbance: animals are recovered on agar petri dishes without bacteria for 2 h immediately after training, then moved to bacterial lawns for 14 h. Quiescence was measured during the period off food.

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