Microscopy from Scientific Research

Time-lapse imaging of newly synthesized CX43 protein incorporated in gap junctions. HeLa cells expressing CX43-SNAP f were first labeled with 1 M BG-555 for 30 min, washed twice with fresh medium to remove the unbound fluorophores, then labeled with 1 M BG-F485 and consecutively imaged. Scale bar, 5 m.

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