Microscopy from Scientific Research

Multi-color in vivo pulse-chase of SNAP f synthesis in mouse livers. SNAP f expressing plasmid was transfected into mouse liver at 0 h and 48 h by a hydrodynamic procedure. The mice received tail vein injection of 40 nmol BG-643 at 38 h and 20 nmol BG-485 at 58 h for SNAP f labeling. Fluorescence imaging of mouse liver samples was carried out at 72 h. Scale bars for the liver, global, local, and 3D images were 5000 m, 1000 m, 100 m, and 100 m, respectively.

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