Heatmap from Scientific Research

Activity heatmap from c , updated on the basis of the results of the directed evolution of a Pyl tRNA specific to class S. S- I2 tRNA Pyl-S52 satisfies all necessary orthogonality requirements (green box). As a result, the S + - Deb PylRS/S- I2 tRNA Pyl-S52 pair requires no further engineering and completes a quintuply orthogonal set of pairs (o.c. 4.0). The data represent the average of three biological replicates. All numerical values and bar charts including error bars showing the s.d. are provided in Supplementary Table 2 .

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