Network from Scientific Research

Schematic summarizing the key results of the N, A and B tRNA Pyl screens. By screening natural and engineered Pyl tRNAs, 18 out of 20 interactions, which need to be orthogonalized to generate quintuply orthogonal PylRS/tRNA Pyl pairs, were controlled (left-hand diagram, the arrows highlighted in blue show the interactions that were successfully controlled). Three fully orthogonal Pyl tRNAs (labelled in green with their cognate PylRS enzymes) were identified, and for each of the remaining two Pyl tRNAs (labelled in red) only one cross-reaction remains to be controlled (right-hand diagram).

DOI