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Open access visualization of Heatmap, Pyl tRNAs, PylRS enzymes, GFP(150AllocK) His6, GFP(150TAG) His6
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Heatmap displaying the activity of combinations of the selected Pyl tRNAs and PylRS enzymes, measured by the production of GFP(150AllocK) His6 from cells bearing a GFP(150TAG) His6 gene in the presence of 4 mM AllocK 2. Values are given as the percentage of wild-type GFP. Only PylRS enzymes and Pyl tRNAs that have greater than 30% activity with at least one tRNA Pyl or PylRS enzyme, respectively, are shown. The data represent the average of three biological replicates. All numerical values and bar charts including error bars showing the s.d. are provided in Supplementary Table 2.

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