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Activity of each combination N PylRS i and N tRNA Pyl j , plotted against the sequence identity between N tRNA Pyl i and N tRNA Pyl j , where N tRNA Pyl i is the tRNA Pyl from the same organism as N PylRS i . N Pyl tRNAs with greater than 75% sequence identity (dashed grey line) are predominantly active with each other’s synthetases (93% of cases). N Pyl tRNAs with less than 75% sequence identity may or may not be active with each other’s synthetases. Dots represent the mean of three biological replicates; error bars are shown in Supplementary Fig. 1 . All numerical values are provided in Supplementary Table 2 .
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