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Heat map illustrating the relative activities of substrates 13 – 15 with Ma FRS1 and Ma FRS2, as determined by intact tRNA analysis (Supplementary Figs. 13 – 16 and 25 – 30 ) as described in Methods . The reported yields are percentages based on intact tRNA analysis. The black box indicates that no reaction product was detected. Initially no acyl-tRNA was detected when Ma FRS1 was incubated with 13 , but when the enzyme concentration was increased fivefold, acyl-tRNA was detected with mono- and diacyl yields of 0.7 and 9.7%, respectively.
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