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LC–HRMS analysis of Ma -tRNA Pyl acylation reactions using Ma FRS1 or Ma FRS2 following RNAse A digestion. Adenosine nucleoside 12 acylated on the 2′- or 3′-hydroxy of the 3′-terminal ribose of Ma -tRNA Pyl could be detected in Ma FRS1 and Ma FRS2 reactions with α-thio acid 13 , α-carboxy acid 14 and N -formyl- l -Phe 15 .

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