Workflow from Scientific Research
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Experimental paradigm for gene knockout in cortical neurons derived from hPSCs: Cas9 expressing neurons at d25 were infected with lentiviruses encoding gene-specific gRNAs. Induction of precocious maturation was assessed by western blot (WB) at d35 and Ca 2+ imaging at d40. Scale bar, 50 m.
#Workflow#Microscopy#Experimental Paradigm#Gene Knockout#Cortical Neurons#hPSCs#Lentiviruses#Western Blot#Calcium Imaging
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