Effect of gene knockdown on GLUT4 trafficking and glucose transport at 0.5 nM insulin stimulation in wild-type 3T3-L1 adipocytes (n = 3 biologically independent replicates, 2 technical replicates per n). Genes are clustered using hierarchical clustering. All results are normalized to nontargeting control. Gray represents genes for which glucose transport data were not collected as their role is established in the literature. Scale represents relative effect of gene knockdown compared to nontargeting control for given measures—red positive, and blue negative, with the maximal scale representing the maximum absolute response relative to nontargeting control (0) across all measures, expressed as a percentage, to allow direct comparison across these.
