GJ plaque formation reduced in cells expressing mutant CADM1. Quantification of GJ formation in H295R cells cotransfected with GJA1-mApple and either WT ( n = 212) or mutant CADM1 (mutant, n = 291) vectors tagged with GFP. GJ communication was detected by internalization of GJA1-Venus in cells coexpressing GJA1-mApple and GFP, shown as a percentage of mApple and GFP expressing cells. Statistical analysis was performed using two-sided Fisher’s exact test. *** P = 0.000205; 95% CI, 1.423–3.147. The statistics used to produce these plots are provided as Source Data Fig. 4 . CI, confidence interval. WGA, wheat germ agglutinin.
