GJ-mediated communication reduced in cells expressing mutant CADM1. Quantification of the experiment performed in a . The percentage of calcein red-containing cells within a 50-μm radius from an injected cell (green nucleus, marked by orange WGA dye in the cytoplasm) was calculated per total number of cells within the radius. There were up to twofold fewer red cells around a CADM1 variant transfected cell compared to WT transfected cells. Center line represents the median. Upper and lower bounds of box represent interquartile range. Upper and lower whiskers represent maximum and minimum values in the range, respectively. Statistical analysis was performed using one-way ANOVA ( F = 20.68, P < 0.0001) and Sidak’s multiple comparison test. * P = 0.028, ** P < 0.0001, # P = 0.0120, ## P < 0.0001, † P < 0.0001. The number ( n ) of dye-injected cells per experimental group is shown in parentheses.
