CADM1 mRNA expression ( c), CYP11B2 mRNA expression ( d) and aldosterone production ( e), each in human adrenal cells overexpressing WT or mutant CADM1 or silenced for CADM1. Transduction of CADM1 (G379D or V380D) as its short (442 amino acids) or long (453 amino acids) isoform increased CYP11B2 (encoding aldosterone synthase) and aldosterone production. No effect was seen with overexpression of WT CADM1 , whereas a decrease in CYP11B2 expression and aldosterone production was seen with silencing CADM1 (shCADM1). All values are expressed as fold change relative to EV or nontargeting control cells. Data are presented as mean values s.e.m. n = 3 independent wells. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test for overexpression experiments. For CADM1 mRNA expression, F = 49.00, P < 0.0001; for CYP11B2, F = 158.4, P < 0.0001; for aldosterone, F = 149.0, P < 0.0001. Two-sided Student’s t -test was performed on silencing experiments. When compared to WT or sh CADM1, * P = 0.0130, ** P = 0.0029, ***P = 0.0003, **** P < 0.0001. NS, not significantly different between WT and mutant CADM1. The statistics used to produce these plots are provided as Source Data Fig. 2. UT, untransduced cells.
