E14.5 WT (a - d) and cRE1 dup/+ (e - h) hindbrain sections at r4 (a, b, e, f) and r6 (c, d, g, h) axial levels showing immunofluorescence with ISL1 (blue) and GATA3 (green) (a - h) together with GATA2 (red: a, c, e, g) or NR2F1 (red: b, d, f, h). Ectopic dorsal r4MNs are present in e and f compared with a and b. Dotted yellow and blue rectangles (a, b, e, f) surround IEE VEN and OCN regions, respectively, and are magnified below. Dashed white squares (c, d, g, h) surround facial nuclei and are magnified below. White arrows show OCNs and white arrowheads FBMNs. The borders of the hindbrain are outlined in gray. Scale bars, 200 μm (a, c) apply to a, b, e and f, and c, d, g and h, respectively (n = 3 (a, c), 3 (b, d), 8 (e, g) and 7 (f, h) embryos).
