Growth curves of C. aerofaciens strains containing GalNAc pathway genes on medium supplemented with different sugars. The x axis indicates the hours after culturing in the medium. The y axis indicates cell density measured as the OD 600nm value. Points on the growth curves represent means s.d. of three replicates. YCFA medium and YCFAglucose (Glc) medium were used as negative and positive controls, respectively. GalNAc and galactose (Gal) were supplied in YCFA medium to test whether C. aerofaciens can grow with the monosaccharides released from A-antigen and B-antigen as their sole carbohydrate sources.
