Control (n = 5 mice), SELECTIV-KO-Alb (liver-specific) (n = 5 mice) and AAVR-KO (n = 5 mice) mice were injected with 3 x 10 10 vg AAV9-luciferase by systemic (i.v.) injection (both male and female, 6–8 weeks of age). Transduction was tracked over time by in vivo imaging and assessed ex vivo. Transduction was measured ex vivo for the liver, heart, lung and muscle, which demonstrated specific transduction of the liver in the SELECTIV-KO-Alb mice, with detargeting of the other organs and tissue. Mean value and s.e.m. are shown. The horizontal dashed line indicates background luminescence in lysates of a mouse not transduced by AAV9-luciferase. Fold-changes are indicated, and the P value was calculated using an ordinary one-way ANOVA with Holm–Šídák’s multiple comparisons post-test, with **** P < 0.0001, * P < 0.05 (control versus SELECTIV-KO-Alb: liver P = 0.075, heart P = 6.5 x 10 -9 , lung P = 3.2 x 10 -5 , muscle P = 9.4 x 10 -8 ; SELECTIV-KO-Alb versus AAVR-KO: liver P = 5.5 x 10 -6 , heart P = 0.020, lung P = 0.34, muscle P = 0.012).
