Control ( n = 6 mice), SELECTIV-KO-Myh6 ( n = 8 mice) and AAVR-KO ( n = 6 mice) mice ( n = 4, 6, 2 mice, respectively, for muscle) were injected with 3 x 10 10 vg AAV9-luciferase by systemic (i.v.) injection (both male and female, 6–8 weeks of age). At 21 d after injection, mice were euthanized and luciferase activity was determined in indicated organ lysates to determine AAV transduction efficiency. Mean value and s.e.m. are shown. The horizontal dashed line indicates background luminescence in nontransduced controls. Fold-changes are indicated, and the P value was calculated using an ordinary one-way ANOVA with a Holm–Šídák’s multiple comparisons post-test, with **** P < 0.0001, *** P < 0.001, ** P < 0.01 and * P < 0.05 (control versus SELECTIV-KO-Myh6: liver P = 9.0 x 10 -6 , heart P = 7.9 x 10 -5 , lung P = 0.00051, muscle P = 1.0 x 10 -5 ; SELECTIV-KO-Myh6 versus AAVR-KO: liver P = 0.16, heart P = 4.3 x 10 -11 , lung P = 8.9 x 10 -7 , muscle P = 0.11).
