Line Plot from Scientific Research

Akt1 pT308 across all the samples and Akt1 substrates phosphorylation levels profiled by Optop-DIA. Left panel: The extracted ion chromatography (XIC graph) from DIA analysis for pT308 is used to infer the quantitative changes between samples. In the XIC, the peak groups above the middle line denote the MS2 level ion traces (i.e., peptide fragments) for the phosphopeptide signature, whereas the peak groups below the middle line denote the MS1 level ion traces (i.e., isotopic m/z of the intact phosphopeptide). Middle and right panels: The XIC graphs for the other four Akt1 substrates based on respective phosphopeptide signatures. The arrows indicate the identical Akt1 activation conditions as illustrated for Akt1 pT308 in the left panel. For ( f , g ), n = 2 biologically independent samples. For ( b , d , e ), two times of WB experiments were repeated independently with similar results. Source data are provided as a Source data file.

DOI