Gel from Scientific Research

Removal of poly-Ser-ADPr on automodified h PARP1 in the presence of h HPF1 and poly-Ser-ADP-ribosylated histone H3/H4 tetramer by human and Drosophila (ADP-ribosyl)hydrolases. The reaction was performed as in Fig. 5A , except histone H3/H4 tetramer was used instead of histone H3 peptide. Lower panel shows CBB stained SDS-PAGE of the proteins. The experiment was repeated independently three times with similar results.

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