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Representative images (top) and kinetics (bottom) of EGFP- d Parp and EGFP- d Hpf1 recruitment to sites of DNA damage induced by 405 nm laser irradiation, in Drosophila S2R+ cells. Scale bar, 2 m. Data from B are a representative of 3 independent replicates (610 cells per replica per condition) with n = 24 cell for EGFP- d Parp and n = 22 cell for EGFP- d Hpf1 and represent normalised mean values ± SEM. Sites of irradiation are indicated by yellow arrows.
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