Plots of log 2 fold changes in miRNA levels observed upon loss of ZSWIM8 in all mammalian and fly cell lines examined in Shi et al. [ 10 ]. Two datasets were derived from CRISPRi knockdown (KD) of ZSWIM8 in K562 cells, using one of two different guide RNAs (A or B). All other datasets were derived from knockout (KO) of either ZSWIM8 or Dora , the Drosophila ZSWIM8 ortholog. Points for miRNAs that met the common p BBUM significance cutoff of 0.05 are in red, with n indicating the number passing this cutoff, shown as fraction of the total number of miRNAs and passenger strands quantified. Points for passenger strands of these miRNAs are in cyan, if the passenger strands were both annotated and observed above the expression threshold. Points for miRNAs with classifications differing from that of previous work [ 10 ] are outlined in black ( n =7). Points for miR-7-5p, a known TDMD substrate [ 16 ], are labeled.
