The EGFR inhibitors dacomitinib and AZD8931 and the senolytic compound navitoclax exhibited senolytic activity in HMECs. Proliferating HMECs (PD ~ 12) were treated with DMSO or 2 uM triapine for 3 days to induce proliferating or senescent phenotypes, respectively, as in our previous work [ 53 ]. Proliferating and senescent HMECs were then treated with DMSO (negative control), 100 nM/500 nM dacomitinib, 100 nM / 500 nM AZD8931, or 100 / 500 nM navitoclax for 3 days, after which cell viability and live cell number were measured by trypan blue staining. The live cell number was normalized to the number of live cells present at the time of drug treatment. * and ** represent p < 0.05 and 0.01, respectively, compared to the senescent DMSO control calculated by Student’s t-test
