Microscopy from Scientific Research

Ciliary axoneme of quiescent hTERT-RPE1 cells transfected with control (siNC), CCP5 (siCCP5), or CCP6 (siCCP6) siRNA or both siRNAs were visualized by immunoreactivity for acetylated-tubulin (Ac-Tub, green) or ARL13B (red). Depletion of either CCP5 or CCP6 increased the ciliary length compared to the control, but co-depletion of CCP5 and CCP6 did not lead to further increase in cilia length. Scale bars: 2 m

DOI