Statistically significant correlations were observed between sequencing data and laboratory tests for 10 out of 14 major GI community pathogens in the United Kingdom. Results where at least one statistically significant correlation was observed are shown. All correlations, whether significant or not, are displayed in Additional File 4 : Fig S2. No statistically significant correlation was found between the sequencing and diagnostic test for Astrovirus, E. histolytica , Giardia or V. cholera . The darker the colour of a quadrant in a heatmap, the stronger the correlation (coefficient) between the detection of a pathogen in sequencing data (metagenomic or metatranscriptomic) and a laboratory result (Luminex or Traditional). Asterisks in quadrants indicate the statistical significance of correlations as follows: *: p < 0.25; **: p < 0.05; ***: p < 0.01; ****: p < 0.001
