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Phosphorylation of PGK1 at Y194 promotes glycolysis based on the detection of Vmax of 3-PG production in vitro. Wild type PGK1 (WT) and mutant PGK1 (Y194F) were overexpressed in HaEpi cells for 48 h, respectively, and then incubated with insulin (5 g/mL) for 3 h. PGK1 proteins were purified by NiNTA agarose beads to detect the Vmax of 3-PG production. * p < 0.05, n = 6.

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