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20E dephosphorylates PGK1 via PTEN . dsRNA knockdown (500 ng/larva administered in the sixth-instar at 6 h, three times over 24 h intervals), followed by incubation with 2 M 20E for 3 h. PGK1 proteins from the fat body were purified by CNBr-activated Sepharose 4B with the anti-PGK1 monoclonal antibody. p < 0.05, n = 3.

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