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Levels of PGK1, PGK1 phosphorylation, and PGK1 acetylation in the fat body from 5F to adult stages. PGK1 proteins from the fat body were purified with CNBr-activated Sepharose 4B and an anti-PGK1 monoclonal antibody, followed by in vitro detection. 5F, fifth-instar feeding larvae; 5 M, fifth-instar molting larvae; 6th-6 h to 6th-120 h represent sixth-instar larvae at the corresponding hours. P-0 d to P-8 d denote 0- to 8-day-old pupae. F, feeding; M, molting; MM, metamorphic molting; P, pupae. An additional band at about 40 kDa appeared in pY immunoprecipitation indicated as Unknown. n = 3.

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