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qRT-PCR detection of PGK1 expression in the larval fat body based on insulin injection over time and dosage. p < 0.05, n = 8 or 9. DMSO or PBS was used as a solvent control for same time. 500 ng/larva of 20E or 5 ug/larva of insulin was injected into sixth-instar larvae at 6 h. Bars indicate means ± SD for more than three independent experiments with five larvae per replicate. Statistically significant differences were calculated using one-way analysis of variance (ANOVA, p < 0.05) tests
#Bar Plot#Error Bars#qRT-PCR#PGK1 expression#Larval fat body#Insulin injection#Dosage#DMSO#PBS#ANOVA
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