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A nicked duplex modified with streptavidin was incubated with different amounts of nuclear extracts from CAL51 MUS81-KO (lanes 2 and 3) or WT CAL51 (lanes 4 and 5) cells at 37 C for 1 h. The reaction was also performed with recombinant MUS81-EME1 complex (2.5 nM, lane 6). The small arrow indicates an unspecific band, most likely the product of the removal of the biotin moiety from the residual oligonucleotide without streptavidin.

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