Line Plot from Scientific Research

Mus81-Mms4 (M-M), as well as MUS81-EME1 (M-E1) and MUS81-EME2 (M-E2), efficiently cleave the trypsinised 3' flap substrate (left). Quantification of the activity (right). Only the bands corresponding to the oligo 7 - bearing the small TOP1 peptide were quantified. The trypsinised substrates were incubated with increasing amounts of MUS81 complexes at 37 C for 15 min. The reaction was stopped by adding SDS and resolved by denaturing PAGE

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