Gel from Scientific Research

Neither MUS81-EME1 ( A ) nor TDP1 ( B ) can cleave the native TOP1 substrate. The reaction was stopped with SDS in both cases, and the samples were run on native PAGE. The substrate bearing native TOP1 covalently bound to the 3' end of the nick was mixed with the indicated amounts of MUS81-EME1 or TDP1, and the mixture was incubated for 15 min at 37 C for MUS81-EME1 or at 30 C for TDP1. Neither MUS81-EME1 nor TDP1 can cleave the native TOP1 substrate. The reaction was stopped with SDS in both cases, and the samples were run on native PAGE

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