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Relative mRNA levels of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, and NOS2 were determined by real-time PCR and normalized to 18s. Data represent means ± SEM; * P < 0.05; ** P < 0.01; *** P < 0.001; by unpaired Student’s t test. The data shown are representative of three independent experiments
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