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Maternal dams were fed either a WD or ND for 8 weeks before mating and continued through gestation and lactation. Post-weaning pups were subjected to WD and ND to generate four groups: N-N, N-W, W-N, and W-W groups. At the age of 8 weeks, four groups of pups were treated with TNBS to induce a CD-like colitis model.

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