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An optimized protocol for generating cfDNA sequencing libraries enables high-throughput methylation characterization. The key improvement was the optimization of specific end-repair, a-tailing, and ligation conditions to maximize the number of cfDNA fragments available for nanopore sequencing.
#Workflow#Illustration#cfDNA sequencing libraries#Methylation characterization#End-repair#A-tailing#Ligation#Nanopore sequencing
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