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Plasma samples were collected from 10 healthy donors, cfDNA was extracted using QIAsymphony DSP Circulating DNA Kit (QIAGEN) [ 41 ], and independent sequencing libraries were made using 9 different kits (Fig. 2 and Additional file 1: Fig. S1). PE 150 bp whole-genome sequencing was performed on Illumina NovaSeq 6000 sequencer.

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