Relative expression of novel priming regulators in natural variants. (Top) The average GBM level of GPK1 and BRL3 in GBM variants and demethylated variants. (Bottom) Expression of GPK1 or BRL3 under mockDC condition was normalized to 1. Six natural variants and Col-0 were grouped by their GBM level at GPK1 and BRL3 . Col-0, Kondara, and UK-1 maintained GBM at both genes, and Ann-1, Bik-1, Kas-1, and Kro-0 lost GBM at GPK1 and BRL3 . RT-qPCR data from three replicates per natural variant were averaged ( n =9 for GBM variants, n =12 for demethylated variants). Yellow color indicates demethylated variants with non-synonymous mutation in each gene (Bik-1, Kro-0 in GPK1 ; Ann-1, Kas-1 in BRL3 ). Error bars indicate standard deviation. p -values by Student’s t test
