Percentage methylation, chromatin accessibility and gene expression of immune-related genes. Heatmaps showing the percentage methylation (left) and chromatin accessibility (middle) at promoters (defined as 1000 bp upstream and 500 bp downstream of TSSs) of immune-related genes and their corresponding expression (right). Bars on the right show the distance of the TSS of each transcript to the nearest CGI. Black bars in the methylation heatmap represent samples where fewer than 5 CpG sites covered by a minimum of 5 reads were found at a given promoter. SIRPA denotes SIRPa and gdT cell denotes gamma delta T cell. This figure also highlights that, consistent with previous reports, the number of CD4+CD8+ double positive T cells is likely small given the expression of CD4 and CD8 are largely specific to the respective cell types [ 34 ]
