Gel from Scientific Research

TIRP1 outcompetes TIR1-TIR2_R for binding to TIR1-TIR2_R in a concentration-dependent manner in N. benthamiana leaves, as determined by coimmunoprecipitation. A series of Agrobacterium concentrations of TIRP1 -GFP are shown (OD600 = 0, 0.5, 1.0, and 1.5) and co-inoculated with a constant concentration of TIR1-TIR2 (OD600 = 1.5) into leaf tissues; GFP was used as a negative control. Blotting of IP groups from different lanes were measured by ImageJ, and the ratio between the maximum values (relative intensity) is represented in the histogram. Three independent experiments were analyzed, and between-group comparisons were made using Student’s t test

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