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HR phenotypes associated with co-inoculation of TIR1L_R-YFP-HA and TIR2L_R-YFP-HA and that of their variants (1AEM and 2AEM disrupts the AE interface of TIR1L and TIR2L, respectively; 1DEM and 2DEM disrupts the DE interface of TIR1L and TIR2L, respectively). TIR1-TIR2-YFP-HA was used as positive control (top). Evaluation of protein expression using a HA antibody, and immunoblotting of plant actin with alpha-actin was used as a loading control (bottom).
#Photo#Gel#HR Phenotypes#Co-inoculation#TIR1L#TIR2L#YFP-HA#AE Interface#DE Interface#Protein Expression#HA Antibody#Immunoblotting#Plant Actin#Loading Control
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