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Salicaceae CENH3 genes. The phylogenetic tree (left) was constructed by CENH3 genes in assembled genomes as well as the published genome of P. trichocarpa and S. purpurea , and branches are color-coded according to CENH3 type. The schematic illustration of Salicaceae CENH3 genes (right) are shown as boxes representing exons. The two copies of CENH3 gene are identified in willows, while the CENH3-2 loses exons at the N terminus and became dysfunctional in poplars.

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