Heatmap from Scientific Research

Csáky assay heatmap to determineN-hydroxylation of various NMOs toward all substrates. Reaction conditions: 1 mM substrate, 50 mM NaPi buffer pH 8.0, 10 U/mL glucose dehydrogenase (GDH), 10 mM glucose, 1 mM NADP+, 1 mg/mL catalase, 30 μM NMO, 100 μL reaction volume, incubation at 25 °C, 1 h. Data were obtained from triplicate measurements by means of Csáky assay results using hydroxylamine as a standard for a calibration curve.

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