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The decryptM approach described by Zecha et al.1uses mass spectrometry-based profiling of post-translational modifications (PTMs) to study drug mechanisms of action. Cell lines are treated with multiple concentrations of a therapeutic and processed for mass spectrometry. Enrichment of the PTMs of interest is performed prior to analysis with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The concentration-resolved data allows for the generation of a drug response curve for each detected PTM, thus generating hypotheses regarding the potency of a compound to regulate specific PTMs. Figure created using BioRender (https://biorender.com).

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