Microscopy from Scientific Research

2-color 3D dSTORM image of VGlut (green) and Bassoon (magenta) in thestratum oriensof a P22 mouse brain. Middle panel shows a zoomed-in view of four VGlut boutons (green) and their corresponding Bassoon-labeled AZ puncta (magenta). Right panel shows the clusters identified by the point cloud analyst (PoCA) approach used for automatic segmentation of vGlut and Bassoon, with the overlap between segmented clusters shown in orange.

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