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Small G-protein linked immunosorbent assay (G-LISA) assay detecting Rac1 activation in human T lymphocytes nucleoporated as in (D). Fold increase of RhoA or Rac1 activation was normalized to intensity in untreated controls. Data are mean +/- SEM (n = 3; unpaired Student’s t test;*p < 0.05, versus scrambled siRNAs).
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