Characterization of the two classes of lagging chromosomes: early (spindle periphery-localizing and NPC-positive) and late (central spindle-localizing and NPC-negative). Top, delay in NE recruitment on lagging chromosomes relative to the IC (seeSTAR Methods). Sample size: early, n = 38, 17 experiments; late, n = 25, 17 experiments. Center, the distance of lagging chromosomes to the ER-spindle border (seeSTAR Methods). Sample size: early, n = 23, 17 experiments; late, n = 25, 17 experiments. Bottom, the percentage of lagging chromosomes scored as positive or negative for NPCs (seeSTAR Methods). Sample size in the “NPC-positive” and “NPC-negative” groups is indicated (from 17 experiments). Images: single focal plane; time: minutes:seconds; DNA: SiR-DNA; scale bars: 5 μm. Quantifications:****p < 0.0001, two-tailed Mann-Whitney tests; error bars: mean with 95% CI. See alsoFigure S7.
