NPCs are recruited to the IC but not to lagging chromosomes in U2OS cells. Top, time-lapse images of NE (YFP-STIM1) and NPC (SNAP-NUP107) recruitment in a U2OS cell. Arrowheads: lagging chromosome; enlarged boxed regions: NE assembly on the lagging chromosome (yellow) or IC (red). Note: the paucity of infiltrating membranes in the center of the spindle as compared with the IC. Bottom, fraction of the IC (black line) or lagging chromosome (red line) covered by ER membranes (left) or NPCs (right) during NE assembly after aggregate alignment. Zero min. is the time point of maximal ER recruitment on the main chromosomes. Sample size (combined from all time points): IC, n = 84, 18 experiments; lagging chromosomes, n = 39, 18 experiments.
