Actin depolymerization delays NE assembly on lagging chromosomes in RPE-1 cells. Top, time-lapse images of NE (GFP-emerin) assembly in an untreated or DCB-treated cell. Mitotic cells were enriched by block and release from RO-3306 G2/M arrest. Red arrow: NE completion in the NC; yellow arrow or inset: NE assembly on lagging chromosome; asterisk (in the inset): NE completion on the lagging chromosome. Bottom, delay in NE assembly on lagging chromosomes and the IC relative to the NC after DCB treatment (seeSTAR Methods). Sample size (emerin): IC: control, n = 20, 2 experiments; DCB, n = 24, 3 experiments; lagging chromosomes: control, n = 13, 2 experiments; DCB, n = 18, 3 experiments. Sample size (Lap2ß): IC: control, n = 24, 2 experiments; DCB, n = 14, 4 experiments; lagging chromosomes: control, n = 20, 2 experiments; DCB, n = 10, 4 experiments.
