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Same as (C) except that NPC recruitment is measured. Sample size (C and D): HeLa: untreated, n = 82, 7 experiments; Y27-treated, n = 77, 8 experiments; U2OS: untreated, n = 54, 7 experiments; Y27-treated, n = 36, 4 experiments. Images: single focal plane; time: minutes:seconds; DNA: SiR-DNA; scale bars: 5 μm. Quantifications:****p < 0.0001, two-tailed Mann-Whitney tests; error bars: mean with 95% CI. See alsoFigures S1andS2.
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