Representative tSNE plots generated from flow cytometry data of 40,000 host and donor T cells from tumor-bearing lungs collected at day 70. Plots depict combined data from four mice per group with comparable tumor burdens. Percentages of donor T cell types calculated as proportion of donor CD8+T cells; percentages of host CD4+naive (circulating and naive host cells), activ (effector and resident host cells), and Tregcells calculated as a proportion of host CD4+T cells; percentages of naive and activ CD8+T cells calculated as a proportion of host CD8+T cells. As donor T cells differ between groups, proportions as shown allow for ease of comparison between experimental groups.
